Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSVEQNEFLSQVQLQILAEQAIKQYPSDYQGQVKLLCQSENATYQVSTGSARYALRI-HRPNYHSKLDIQSELEWLDALNASGIQVPVAIADQSGERVITLKLSNDIYRYAVLFNWVEGDMPTVEVDP-TAFEQLGQITAKLHAHSKTWQAPENFQRIVWNHETMVG-ADGHWGNWKNAPHLRPQDHGVIEEAIAQISKDLNFFGKTQERYG--LIHADLRLTNLLLQ-----QERIGVIDFDDCGMSWFMHDLAAAISFNEHYANAPHWVDYW-LKGYERVGHIQSEEYEMIPTFIMQRRI-QMMAWNGSHAQTE-----------MAQSLGDQWSNETVRLCKKYLNGQMPVGI--------- 3CSV Chain:A ((4-333)) SREDEIRDFLATHGYA---------------------DWNRTPRYQRLRSPTGAKAVLMDWSPEEGG-DTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLI---EDLGD-----ALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELAR-------LDPETLSEMTRLAFSEYRYAILGDAA-----EDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLL--QDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 8782 6.57 30.39 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 6.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: