Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSNNNNPLTRDEILSRYFPQYRPAVATSQGLSGGSCI-IAHD-THRVVLRRHHDPDAPPAHFLRHYRALSQLP-AS-LAPRALFYTP--GWMAVEYLHGVVNSA---------LPDADELAALLYHLHQQPRFGWR-IA---LSPL----LAQYWSCCDPARRTPFWLRRLKQLQKNGE-PRPLRLAPLHMDVHGDNIVLTSA-----GLRLIDWEYAGDGDIALELAAVWVEDER-----QHRQLADAYAARARIDARQLWRQIRLWHPWVIMLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG 3CSV Chain:A ((4-301)) ---SREDEIR--DFLATHGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPE--EGGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFE---

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1124 -23216 -20.65 -90.69 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -20.65 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: