Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAPFSIKQADIQQQLTAAIQKHFPAVEIADIRFELV-GGLSSKSWRIRGP-GIEWLARRQSPTERKMGVDRQREFALLRRMSAIG--L-APRPLLWRDDWLIVEWVPGRIATSDEF-------------LMMLANGEVARVLSQLHRLPRSGHPL-D-----IKMLFAQHWQLMDPHR-----RSPVLLRAHHYFQRAPLPTPLALAPLHLDVHAENLLITP-QG-TMLIDWEYASDGDIAFELAFIVRASQMDSMAQRYFLQSYQRHRRGFSVNRLQQQMRQWFPWVDYLVLMWFEIRWQQTKNPEFLADIPALYHRLQLGHRLQPD 5IWU Chain:A ((3-299)) ---------KDIKQVIEIAKKHNLFL--KEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDV--MPRTKVEKQALDLVNKYAISFQAPNWIIYTEELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDD-MWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF--GEEGLEALILAYKEIGGYY-WPKMKEHIIELNAAYPVSIAEFAL---VSG-IEEYEQMAKEALEV----------

General information: TITO was launched using: RESULT: Template: 5IWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1186 -31179 -26.29 -117.21 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -26.29 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_5IWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iwu-query.scw PDB file : Tito_Scwrl_5IWU.pdb: