Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSPLQLAAIVSAGVPG-I-----YPVGAEPVREDSDDFDSAIIADST---------GRRWRVRAPRRPDAS--MRLEAEHLILQSFSPGLRARLPFAVPTVAGTVPYAGGNVFVYTHIPGTIYDVDQLAELSRREVAARRPSLASIIAKDIATLHVMPEDIIYEADLPSYSSEQIRLRKNAELERAAA-T----GK----VPA---DLLAHWRAVLSPGPMWQFRPRVVHGDMGAENLVLHVTPTEA------RIVEVTGWSEVHVGDPAQDFAWLYSCQD--------------------IDFTDEAIEVYRQQMPQL-----------PDAYLAQRANFYAEFAIAQWLTHTVEVGD-----RDGATHAVSMLLDIQDDLRASGELLGQEEVGPLVGPAE 5FUT Chain:A ((8-377)) ---TRRRAYLWCKEFLPGAWRGLREDEFHISVI--RGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILA-----ERSLG-PKLYGIFPQ----GRLEQFIPSRRLDTEELS----------LPDISAEIAEKMATFHGMKMPFNKEP--K--WLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES---TPSPVVFCHNDCQEGNILLL----EGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLG----------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1056 -15112 -14.31 -54.75 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -14.31 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: