Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSHLTHIWRLLKWGRTLARHGALTGIERDPLTPTPVRRLVRVARLGARVPRQPRYADAFQSIGPAAIKLGQTLATRPDLVGEDAANDLLRLQDALPPVPFATIRAQMEQSFGR-PLETLYSRFDEAPVGAASIAQVHRAVTT-DGRDVAVKVIRPGVIDQFNRDIQTYEWAAAHVEMLGGEVARLRPRLVIANMKRWTARELDLRREAASASELGEAMEAMPGYRVPAIDWDRTTGKVMTMEWIDGVKISDRDALIA-AGHDVKEIAARLVNAFLRQAIAEGFFHADMHQGNLFVTANGDIVAIDFGIMGRIDRRARMWLAEILYGLITGNYRRVAEIHFEAQYVPGHHNVEEFATALRAVGEPMRGKPVRELSVGGMLDGLFAITRDFDMQTQPHLLLLQKTMVMVEGVATALDPDINLWETSGPYVKEWLRAELGPEAKAADAL-IENVRILQRLPGLVKRIEEAFPEKGGAPPPPPLTEVRLIRVGSGWRYMLVALLAGLAGAGAMAAATTFL 2Q0N Chain:A ((8-282)) -------------------------------------------------------------------------------------------------RVSHEQFRAALQLVVDPGDPRSYLDNF--IKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRR--------------E--------------------------LLFNEVVIMRDYQH-----ENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQA-LSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPR------RKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGE--PPYFNEPPLKAMKMIR---D----NLPP-----------------RLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFL-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1382 -13902 -10.06 -51.30 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -10.06 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_2Q0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q0n-query.scw PDB file : Tito_Scwrl_2Q0N.pdb: