Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFENESMGFNHDEICATWNIPPLVSVQKPL-TGTIHNVFLLTTREAKYVLRIYS--YPPEKRFRIVNEHAIARYVQSHHLPAIAPLPISSGCETFLERDGHFYALYPFAQGEQLSRAMLNSNQSHVGEIISAMGHSLAEVHLVLSSYCLPTTRPLSLVVNREQTIAKIEELEIVIPAKEA--LDDLDQRVLSALRARKQYLLTASDVDVSELNALPWQPLHGDFQETNLFF--SHGRVSAIIDWDQACSGPRAWEILRTLHYV--FALDPSRCQR-------FLEAYQQVFPLPM------EVLHLTAKVYTWVQLNNLWGWRSYYLDNNQSLRQLLSTSDSQPFTTRWAQLAKML----------------- 4OCV Chain:A ((24-378)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSG-DTWAEIDGGAWRVYKFIEH------TMSYNLVPNPDVFREAGRAFGDFQNFLSGF--DANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRD---GSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1532 -15312 -9.99 -48.46 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -9.99 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: