Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSYAHLNHYISPSAEAGLSDAWRRSLGRLGDYSAILIRSLNLRPLEVSLHDQTEEHILVRIRLPGSYLILRVAPED---DLAAYVYQIRTLNGH----NIPAPQIIQRDLSRSLVPFAFTLESYVPGVTAIELQSAP-LLRGAGRQAGRALRRMHRIPVTSAGRPTASGRWTQQNWRTVLRQIGRRLAAPPADALIFGEREQRVVADLLD-----------HPVLECQQPSLIHGRFSPRAVRCTSSGNVHVEALIDPGHYVGGDGLYDLACGLCAAYPEPWREGLLDGYQSAGPLNEAERRRLPLLQLLTNYWEACRLYMHAEPHEAARSEAIRLMEEQVGLTRLVSV--------------------------------------------------------- 2OLC Chain:A ((5-396)) ------------------------KTPLYETLNESSAVALAVKLGLTLTCQEIGDGNLNYVFHIYDRALIIKQAVPYWPLTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAETLIHGDLHTGSIFASEHE----TKVIDPEFAFYGPIGFDVGQFIANLFLNALSR---DGADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKLLVK

General information: TITO was launched using: RESULT: Template: 2OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1385 -53351 -38.52 -177.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -38.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_2OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2olc-query.scw PDB file : Tito_Scwrl_2OLC.pdb: