Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDLDKPIAYGRTAEIFAWGE---NQVLKLFHAWV-GFDAVEYEYRIARTMQS-CGLPVPQVGEIIEY----------KGRYGITCQRIKGISMTESLARAPWKILRYARRMAELHARVHAVTHPTDIP--PL--NPK------MARKILQA--QSLPAR----LREKALTRLEK---LPEGHDLCHGDFHPDNILLTP----QGEVILDWMDAGTGNPLADLARTSIILKGSIESGQIRHPLLKRLAHLFHRAYLAQYFSLRPGGESEYALWLPIIAAARLSENISELEGWLVAQAERGLS 6EF6 Chain:A ((50-307)) ---------NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEES-LTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD-P-----------RLGEWQESWVAGYRSRRELPAADEAMLPSFV-------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 -11369 -12.82 -52.39 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -12.82 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: