Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQVSEKDVTEAINQLGPENHIEEKEDISGHNN-KVFRIKLEERTVICKFCTGENSIQDCRKEVNMNQLLKNQ--TTIDTREILYSNSSTEKTEFPFFITEYIEGESLQD-SFTELTAEKQVEIIEQAGQILGEVHSKISFQYAGELIPENSNISVNGKTTWIEYITQELTEAVKKAEYTRFSDLTEKAENLVEA-LSKLEEPERTLVFYDFRPDNVIAES-SRIKALIDFERALSGDPFWDYAYSEMSFVEPHQYYNMHEPPDADEEKLRKAFQRGYEKQMELKENWRQNVKLYKLGILIKRFNSFKGWTESAEMTEEEIRRQEKLLKKGFDKLYQSLDIQ 5IQI Chain:C ((9-281)) --ATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN---NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISD---ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL-DYTDISECTIDNK---QNVLEEYILLRETI----YNDLT-DIEKDYIESFMERLNATTV--FEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI------------GTNFGEDILRMYGNI--DIEKAKEYQDIVEEYYPIETIVY------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1164 34537 29.67 129.35 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 29.67 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: