Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIR-R---SELAAQEVAYHNTDRSIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFE-------THKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKE-EQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 5K00 Chain:A ((16-265)) ------------------------------------------IGTG--AKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLR--HQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYV---------------------------------------------------------------------HSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKP------------SL---AYAAPELIQGK----SYLGSEADVWSMGI-----LLYVLMCGFLPFDDDNVMAL----YKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNL-LNHPWIMQD--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5K00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 980 23566 24.05 109.61 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 24.05 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_5K00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5k00-query.scw PDB file : Tito_Scwrl_5K00.pdb: