Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESTDEPGDTVDTELFTRVVRHGLPDVTVETVTPGGPSWNPVTEVARVGFADRDPVYCKAAPGDH-EGDLRAEAGTLDYVGAT--LRVRVPSVVRVT-TEPVAALLTEPVAGRAVAD-EWFETTPERRATLAERLGRTLATVHTERFDRAGEITGGDPDGLALDHAPWPEVLRAGVTETQRRAPTDRFDPECARLLDAIDDSRDRLADPPARLLHRDPATPNCFDTGD-DRLTLLDWGNSVVGDPVCDLVRAREQVLAPSREPAPDRLVAPLRRGYRAVAGGVPSAADREPVYEAAITLSTAGFVDRLAEWREESVAELTAWFRAELDGRLSAVE 5IQC Chain:A ((5-281)) -----YDDNATNVKAMKYLIEHYFDNFKVDSIEII--GSGYDSVAYLVN----NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTI------DNKQNVLEE-YILLRETIYNDLT-DIEKDYIESFMERLNAT--TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI----GTNFGEDILRMYGNID--IEKAKEYQDIVEEYYPIETIVY-------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1201 7339 6.11 27.08 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 6.11 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: