Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSPKLKLNLYQKLLGLDH-AQFSLIQHEDAIVAIVYEVIEPSGKKLILKICEQE---RHFLREVYFLEALKD--KIPVPQILQAIEPNS---KRHGAILMEYLPGQVLNKE---TLTCELAEKAGYQLARLHLNSEKGYGDLISKDSLTQDPKADLIQRFEEGLDECEAHLPSSLLNECRHYFHTHLYLLELADGPCIIHRDFRPGNLLANK-NKLLGIIDWASARAGFAQEDFSSLEMGEWSTQASIQAAFLSGYETIRPIPRYQELMPLLKLSRSAAILGYLYRKGIYHKSSAKLHQSHLNFLKTFIEA 5IGI Chain:A ((17-257)) ------------ARHGLKLHG--PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV--VNDKRLHRWQRWLDDD---SSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMV---FGEEGLAKLLLTYEAAGGR-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 929 -18502 -19.92 -81.50 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -19.92 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: