Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAACPELVDATFAILASGWHSTAVEVG----GRWVFKFPRGAEAEAALLREAAVLRLVRPA-VSLPVPAPQVFAGPPLFSRHEKLPGGYLLGEDYRR-------LDEPARQAVGDALGRFYAELHRLPPARMRAVGAL------PVRPWESP-AAMRRRALPLLPAAWRERAERLVREYAKLP---ADPLGSIFGFFDGHGWNMAFDSEAGRLNGVYDFADAGIGPLHQEFIYSA-FIDADLTERIVAAYERFSGRRLERRRI--ALLTA--AHRLSELAELADEPRHLPDMLAGALDGLALAEAQGLA 5UXA Chain:A ((20-294)) -------KEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT--EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRA----KFG--VGENLWNRWQAWLNDDDMWPKKTGLIHG--DVHAGHTMIDKD-ANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAK----------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1041 -14062 -13.51 -56.70 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -13.51 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: