Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVIDVGGEVREGEELDIGAVENWLKNQGVELVGPAVVTQYTGGASNWTYRLKYE-NTNLILRRPPKGTKAKSAHDMAREYMVQKNLAP-YYPVLPKMVALCQDEAVIGCDFYVMERIEGIIPRAKLPPELSF--NEEDVHELCVNVIDKLIELHQVPYENTPLAELGKGTGYCRRQVEGWDKRYEKVHTI--NVP-SFKYVRKWLNDNIPQDSTTCIIHNDWRFDNVILNPEHPTEVIGVLDWEMATLGDPLMDLGSALAYWVEPTDNMIFRSTRRQPTHLKGMFSRKEVVDYYLQKTGLEPQNWTFYEVFGVFRLAVIAQQIYYRYYHKQTRNPAFKDFWIVIHALHIRALKLIAQQKLQESEFAQQSLQKIQGILRR 5UXD Chain:A ((4-255)) --------------PEDLDALLDLAARHGLD-LDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPD---VLERAAVEGRLLAMLAPHLDVAVPDWRIST-------SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVR--SPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDE---RITAVLDWTTAAVGDPAKDLMFHQVSA-P-------------------SAIFEVALQAYAEGGGRP------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1022 -40070 -39.21 -163.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -39.21 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.220

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: