Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MEEALARHDRFGEDFSKVFTIINSAEIPAVENSALYLFVGTSRAPDEASKYVRDRVAQNAQSSTLEETLHSIHEELKILSKKMTREDPMNLDPEIEAALYERD-----GGCCFITGR-----TAGVQPIYIIP--PSILEDKD--LQPGGYLRPLLEVSL-TKEGTEQMLNLLGLPGRENVLKNLILMEPSIRY-SFRHGYFEII---KSPYLEPPYLPTDAPKSKNGGWWLQPIAPQSEVPQIIPRNNELYKVPS------TINPISHPLPARLLLKTH------GI----VSHPLHTIYIEEQIKAGWPVEPEPKELNWVGRLLLQILLRLIPNFARIRLYELIYKMVDYWDPSQKGSHVKFLPLGLVLKKGRENTENEAKALTLAEQYISISTPRLIDSVMVNKTSGFILMTKVVGRSLSSILHRITWEELEQIGKDLANFITELRR-IPNSSKYLIADTRGGPISDHRFFYQTWGPFKTVSGFTDRLLQDVKGARDKPPLSFLYEKKHKVYFTHSDIHMTNLFVTCGRLSGVVDWENAGFKPEYWEYIRAMWAYGADKHAKCLYGSAFGDKYKEEGGIEITAVWIGFSLTTFSGFSGLIFEQLIRLRNEYLPLAQGKIS--PDVNPGHLCSTAKTSYTSAFARWEAVPARPMPTSQSGPITAKDAFKG---------------------- 1G5A Chain:A ((1-628)) SPNSQYLKTRILDIYTPEQRAGIEK-SEDWRQFSRRMDTH-FPKLMNELDSVYGNNEAL-LPMLEMLLAQAWQSYSQR---------NSSLKDIDIARENNPDWILSNK--QVGGVCYVDLFAGDLKGLKDKIPYFQELGLTYLHLMPLFKCPEGKSDGGYAVSSYRDV--NPALGTIGDLREVIAALHEAG----IS--AVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIFPDQHPGGFSQLEDGRWVWTTFNSFQWDLN-YSNPWVFRAMAGEMLFLANLGVDILRMDAVAFIWK-----QMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSEAIVHPDQVVQYIGQ--DECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDD--------IGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFQYNPSTGDCRVSGTAAALVGLAQDDPHAVDRIKLL---------YSIALSTGGLPLIYLGD-------------------EVGTLNDD------DWSQDSNKSDDSRWAHRPRYNEALYAQRNDPS--------TAAGQI-YQDLRHMIAVRQSNPRFDGGRLVTFNTNNKHIIGYIRNNALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVSLNQDLTLQPYQVMWLEIA

General information: TITO was launched using: RESULT: Template: 1G5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3033 68479 22.58 123.83 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 22.58 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_1G5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1g5a-query.scw PDB file : Tito_Scwrl_1G5A.pdb: