Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIISRKYINEGFNSKAYIINDDYILLDGVNQNSYKNYQKYVNVINQISNV--KSLEIPRIIELIEPCEEYPNGALIYKMIKGHTFRKEHIE-IV--NLDNIAKKLAEFMDELYEIRVDFDKDEYIKNELEITEQ---SVIELKEYLSESNYEKILSWFNEYKN-YLLTFNDYHFIHGDLWYENYILNDNNELVGIVDFEGSGMGDPAYDIAALYY-----LGTGFINKVLSYYKYT-DEDLIKRVSMLIKAREIADFDDMVK-NYPEEVEEQVDKIKKVL 5IQI Chain:C ((28-299)) --VDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYIS---DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE-GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIY---

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1123 -14957 -13.32 -58.43 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -13.32 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: