Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANPLDHKIKEYLRKLDPTILDIDNI-------QSISTHKIGLGESNLNYLAIING---------KKFNIRINMYPHFP--KKSENEFNSLKIVEPLGITPKVFHYEASKEILGETFIILEYLDGRSLNKYKKIGGDVIRKLGVAVAQLHNTNINNIENRLKRHGSSKMDLLNIIKQMIEYI-NRKRKHYFPIRGVFENVLEKSYKHLQRLNFPIKPSYVLGHGDIDPSNVISSQ-------RKLKLIDWEDLGLMDPALEIVGLFDSFDL------------------SNKEKELFLKGYLEVRED--------------TSLKKKLPIFLPFQIFRVFCWAIMHIYELGEGETNEVFLKEQDLKEHIDYTEKMFTKCKKAEIIDKDVRWNISEIVPEHYLKLTT 5FUT Chain:A ((4-347)) -------PEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQ-------GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEP-KWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLE----STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIV-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 981 5337 5.44 20.29 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 5.44 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: