Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MSQDEQVRAWLERELDGTVVAFERQERWRAAWFAELKNQGE----LRSYYVRGHRGGN----------------FREMITLGQEADINRV--LHRHGVPVPRVHGMIDDPPAIVMDRLPGRVNLATALDDAE--REAVLDQYVDAMCRMHAIDVNEFGALGLPVPADNRAAALNMYATGERNYRRAKT--APSPMIEFMWRWLCRNVPAGRTQQGLIQADAAQFLFEQGRMTGLID-FEAAYIGDPIAEFAAMRSRDCEEPLGDIGRI-ARRYEAVTGEPVDRDAVEFHTAGWSLMTPFQWEDSVRNPAPG--DSWLEYFTWLVGCGRWALEAIAAVAGAPL----PTVEAPAQRPSSYSSAAYTNLMSLLDEWPTASAYEGFRADSARAVTRFAEHVHA------FGADLEGA---AVEEISELLGTRQPDLATAEAALEQFV-------LRASPDLDVALVQYFHRWTQRQQFLIAGTGARHEKL-------------IHLSL-QPLTADAWQAEGQSRRHA--------------------------------- 4XGC Chain:C ((1-676)) MQPFYEEYRKAWNQINDHIADLQHRSYARTLEQLVDFVVGQAERDEVLPTAALLTGINQPDHLSQFTALTQRLHAQRAAMVCVLQSRDCATLKAAVETLVFGLVEDNAEVEQLRRSQCTMKQLKSWYTNN----FDSEQKRRQLVVILPDFECFNASVLQDLILILSA-HCGSLPFVLVLGVATAMTAVHGTLPYHVSSKIRLRVFQTQAAPTGLNEVLDKVLLSPKYAFHLSGKTFKFLTHIFLYYDFSIHGFIQGFKYCLMEHFFGGNAFALCTDYSKALGRIKQLTHEDMETIRRLPSFRPYVEQINDCKRIIAVLTDDDYLKKKLPQLLRDCLLHFLLFRCSLEFLTELVGDLPRCPLGKLRRELYVNCLNRAIISTPEYKECLQMLSFLSK----DEFVAKVNRALERTEQFLVEEIAPLELGEACTAVLRPKLEAIRLAVDEVGRALQKTLQLIETQIVQDHLRALQDAPPIHELFVFS-DIATVRRNIIGAPRAALHTALNNPHFPDLSVVYKLHLECGRMINLFDWLQAFRSVVPQIQARFTRAVAELQFLGYIKMSKRKTDHATRLTW

General information: TITO was launched using: RESULT: Template: 4XGC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1822 16486 9.05 36.64 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 9.05 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_4XGC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4xgc-query.scw PDB file : Tito_Scwrl_4XGC.pdb: