Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAKTPEFSNAQVIELLLQQYKLSGQLKKLPGYCDQNLLLNTDDGKQYIVKIANAVEPKLELEMQNAAMAHLTHKQCAVPCAVNNIQGDNISIITDVSKQSFCLRVLTFIPGKFYADANSNTHSRALWTDLGQFIGNIDLALADFQHPGAFRYLDWDLAQGYRVCMSKKHLLNPQQVNLVDKFLSLYQTQTMPVLSQLPQGVIHNDANDYNLLIDDINTPNKISGLIDFGDMVHSHIVNELAIACAYALMGEKSVHEDILSALTSIVAGYHKIRPLLDVELEVLYSLIALRLCTTVCNAALAIQQQPDNEYLLVSVQPAWQLLEQLSAVNSYAVLCQLRLACQLPVDSGQSAAEIINYRKKHLGKTLSLSYQQPLKMVRGQGAYLFNEQGVPYLDMVN--NVCHVGHCHPKVVAAGQAQLAKLNTNTRYLHDNI--VNYADKLLATMPDKLS-VCMLVNSGSEANELAFRLARCFTKSRELLVVDGAYHGNTNACIEASPYKFDGPGGEGAPAYVHKVTL---PDPYRGEFLGNNSDAATAYANSVKETIEQLALNNKKPGAFICESLQGVAGQIIMPDGYLTQVYAHVRAAGGVCIADEVQVGFGRVGSHMWAFETQNVIPDIVTLGKPIGNGHPMAAVITTQEIAD---AFVTGMEYFNTFGGNPVSCAIGIAVLDVIEQEQLQNHALTTGQYFQDQLNKLKQRFELIGDVRGKGLFIGVELVEDRTTKQPATDKTSWLIEHFKQHHILLSTEGPFYNILKIKPPLAFNSGDADKFINVLELGLAVLSH 2ZUK Chain:B ((39-439)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLAISGGRGARLIEENGRELIDLSGAWGAASLGYGHPAIVAAVSAAAANPAGATILSASNAPAVTLAERLLASFPGEGTHKIWFGHSGSDANEAAYRAIVKATGRSGVIAFAGAYHGCTVGSM-----AFSGH--------ADGLILLPYPDPYRPY---RNDPTGDAILTLLTEKLA--AVPAGSIGAAFIEPIQSDGGLIVPPDGFLRKFADICRAHGILVVCDEVKVGLARSG-RLHCFEHEGFVPDILVLGKGLGGGLPLSAVIAPAEILDCASAFA-----MQTLHGNPISAAAGLAVLETIDRDDLPAMAERKGRLLRDGLSELAKRHPLIGDIRGRGLACGMELVCDRQSREPARAETAKLIYRAYQLGLVVYYVGMNGNVLEFTPPLTITETDIHKALDLLDRAFSELS-

General information: TITO was launched using: RESULT: Template: 2ZUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2302 -10444 -4.54 -27.34 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -4.54 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_2ZUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2zuk-query.scw PDB file : Tito_Scwrl_2ZUK.pdb: