Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGWLFLEHLKQRL-EEMKW-VPLHVECITGAHAGDIFRFEIINHNGDTEKFIYKEFAEG-----RNNEIEVFKKLENHLQPFTKLV-KWWE----SNPQAILMVDL-GRPLKDDF-MNLPLDSKKKSIVSILQRLSYLHSLKIDQTALTLDAHSITYEWSEWAIDQLKLLNEGHDWASDWSKLIESAYQKLKLGQYTVKGPLTLTHGDPHLENVFCYE---NEIWFIDWEWAAIGSPLRDITILLQDV--YDEELVRYTYRAYKELLDSKKISISNDAYRRDFHHLYVDHTTMMLAWEIDKYFQGFTSEARIKEIVEFKMKEINRVVIEEAHISPW 3N4U Chain:A ((5-282)) -----TFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEIN--------RDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNL-VLDFREKINE-DNKKIKKLLSREL-KGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIP--------TVLEKYRMKEKYWSFEKIIYGKEY-----------------------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1117 20214 18.10 78.05 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 18.10 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.252

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: