Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIRRSELAAQEVAYHNTDR-SIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFE--------THKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKEEQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 6EIM Chain:B ((27-288)) ------------------------------------------LGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFL---------------------------------------------------------------------HSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTP---YWMAPEVVMCETMKDTPYDYKADIWSLGI-----TLIEMAQIEPPHHELNPMRVLLAIAASDPPTLLTPSKWSVEFRDFLAIALDKNPETRPSAAQL-LEHPFVSSITSNKAL--------------------------------------

General information: TITO was launched using: RESULT: Template: 6EIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1007 15172 15.07 61.93 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 15.07 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_6EIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6eim-query.scw PDB file : Tito_Scwrl_6EIM.pdb: