Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITLNYIKNNFANIINIVKPKNQGKLSCVYFADYNDTKIVIKIPMNAGSLSKEIQFYELLKNIELPSPKILSYNYKNIYRIPEYLIMTKLNGAPLHDNCCNDSTKFKIYADIGIYLKMIHSINYFSYSTTFTPY--SCDGVINFNSWIEYLNSEIGVILNKVQLSGIFSVYHVEKFRNHFKKLVNI-D--YN-LVLLHGDLGPDHLYFRHDKFEGIIDPGLSFLGPKEYDLAYMAIYTDPHYFHKIASTYGPYSQELVDSLMLLILSQKILRHFENEKVINIEKFINVISTYKLLD 3W0S Chain:A ((10-235)) --SVEKFLIEKFD-SVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFS--ESLTYCISRRAQGVTLQDLPE--TELPAVLQPVAEAMDAIAAADLSQTSG-FGPFGPQGIG--QYTTWRDFICAIADPHVYHWQ--TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFW-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 930 -57861 -62.22 -263.00 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -62.22 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_3W0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0s-query.scw PDB file : Tito_Scwrl_3W0S.pdb: