Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDATLARSLPALLADLWGL-------EDVDVRPLGGGMNSATCLVTATG--------GDRFVAKWS-SDA--ESLEAGCSAATLLGRQGVRTGEPVGSTAGAPVVAMAGGALALLRHVPGRELDG---DSGHEQQLIGTALATAHTAGCPASDTGPFMSEWLY-PAEDVLAV-----------ATW----LPPALAEIRREYNALPQLTWTQLHTDPAPEAFIHDDAT------GSTGLIDWAGSRRGPALYDVASAVMYLG-------------------GPQQAEQFLSSYADRGPVENDELALLDIFRRFRWAIQASYFAGRIVADDRTGIVDDTDHNTEALARARRGLAEAR 5FUT Chain:A ((7-309)) ----RTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---GAEAMVLESVMFAILAERSLG-PKLYGIFPQ----------GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES-TPSPVVFCHNDCQEGNILLL-EGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQN---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 653 -9343 -14.31 -42.86 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -14.31 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: