Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANPLDHKIKEYLRKLDPTILDIDNI-------QSISTHKIGLGESNLNYLAIING---------KKFNIRINMYPHFPKKSENEFNSLKIVEPLGITPKVFHYEASKEILGETFIILEYLDGRSLNKYKKIGGDVIRKLGVAVAQLHNTNINNIENRLKRHGSSKMDLLNIIKQMIEYI-NRKRKHYFPIRGVFENVLEKSYKHLQRLNFPIKPSYVLGHGDIDPSNVISSQ-------RKLKLIDWEDLGLMDPALEIVGLFDSFDL------------------SNKEKELFLKGYLEVRED--------------TSLKKKLPIFLPFQIFRVFCWAIMHIYELGEGETNEVFLKEQDLKEHIDYTEKMFTKCKKAEIIDKDVRWNISEIVPEHYLKLTT 5FTG Chain:A ((4-347)) -------PEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----EAMVLESVMFAILAERSLGPKLYGIFPQ-------GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEP-KWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLE----STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIV-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 990 622 0.63 2.37 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 0.63 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: