Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKNWERFVKVEKVNIGEVTDIMKNMEKDIKITGYKLISKGQRNTNYMVNTLTDKLFLRICADEN--LS-KNEEAAFKILKE----KITMPRLVLTFSQIVKSQKKRIMIYEYIDSITGDEYLEK--NNRFSEKLLKNIAFILAELHNIKILSLKGINSLPSLKECFEMLMEN-SVLRKRMGIDSIKKLNAVIEKYKEEIEKKRENFLVHCDFKTSNLIMDK-DEKIYVTDWEYLGCGNRYFDIGLFFRYKN-YFTKKDMEIFCEEYNRNAEISLDENWIEMGVLCNIVSLMEMLSREEDAFEKNNDIKNLIEKEIKFLIEK 5IQI Chain:C ((9-257)) ------------ATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNN---EYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIE-YSYIS---DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT-IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 940 -11393 -12.12 -48.07 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -12.12 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: