Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGAGAADATEDAATDATRGTDATRGTDATGGTDGPVPAARTAEEDRLVARVEELVGGRVTAIERQPRWRKAWYLTVDRDGAELALYVRGDKQIDAEPYPGLDREAAILRILERNGVPVPHVHGMSSDPIGIIMDRVPGTRDVAEAADDAQRRGIAEQYMEILAR-MHSIDVAEFAAAGIEVPTTPEGAQLAFVDANERLYRRTKKAPEPLVEWALRWARRRLPTAGNRARFIHGDTGQ-FLFVDGRITC--VYDFEASHIGDPLSDLAGLRTRA--GTEPLGADIEHMIRHYQRVAGTTIDPSAL---SFYTATYMLTAVMALSGPLTE--------------------LRPADQQAEYLTWDLMVRRAML-WAIAEVEGVKIEP---------APPVTLPTGYPARVTTVLEGTVRRMVPATGVDEANQSAALALAQWAGAMVAVGMANVERDLDRAAVLLGHRPADQAAADAELERFVLAAGPEHDLALLEYFAAQTEARVAEAVPMRTRLEQYALPKVVL 4PU5 Chain:A ((16-453)) -----------------------PRGSHMS-----------TAKTLTLEMHLGDLMIGELSFDATADTFAVHYTKDWQQSGFPLS------------PTIPLDGTG-------------------TSNQISMFLVNLLPENKGLDYLIESLGVSKGNTFALIRAIGLDTAGAIAFVPKGALLPET----QLRPIKAEEVIQRIEDPTMWPMEIWD---GKPRLSVAGVQPKLNLFYNGKEFAFAEGTLSSTHIVKFEKYH-HLVINEFITMRLAKVLGMNVANVDIVHF-GRYKALCVERFDRRNIPGEQRVLRRHIVDSCQALGFSVSKKYERNFGTGRDVKDIREGVSFNRLFSLAAKCRNPVAAKQDMLQWALFNLLTGNADAHGKNYSFFMTPSGMEPTPWYDLVSVDMYEDFEQQL-AMAIDDEFDPNSIYAYQLAAFMDGLGLP---RNLLISNLTRIARRIPQAIAEVILM---LPPLDEDEASFVAHYKTQLLARCERYLGFVDEVRDVEV-----

General information: TITO was launched using: RESULT: Template: 4PU5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2079 -39708 -19.10 -99.52 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -19.10 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_4PU5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pu5-query.scw PDB file : Tito_Scwrl_4PU5.pdb: