Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSNNNNPLTRDEILSRYFPQ--------YRPAVATSQGLSGGSCIIAHDT---------HRVVLRRHHDPDAP-PAHFLRHYRALSQLP-ASLAPRALFYTPGWMAVEYLHGVVNSA-----LPDADELAALLYHLHQQPRFGWR-I-ALSPLLAQYWSCCDPAR---------R----TPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTS-------AGLRLIDWEYAGDGDIALELAAVWVEDE--------------------R-QHRQLADAYAARARI------------DARQLWRQIRLWHPWVIMLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG 5FUT Chain:A ((8-351)) ----TRRRAY--LWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKI---------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 903 -38520 -42.66 -159.17 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -42.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: