Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRDALDRAAVTAFLVARHGDAVCDSAIEIRPLPGGLCSEVALVTARLRGAAGARRVTLVAKL-V-----HGTAAREAAVYRRLSRSVGRRFSPELLGAERL----GRGRWLLYLERIEPAHAWPWADSGHTARVLERLARLHEETPQGAALPAWDYEAENLAMA-RDTLSLAERLPRGEVPALARALPALRRVVSALPAMRRQLLAFQPLGRAVIHGDVHPGNVMVRRRDGVVEPVLLDWARARMGSPLEDVSSWLQALGYWEPEARRRHDTLLAGYLSARGLPPALTRPLRDVYWFASASNALAGALGHHLAMACGASAPGVLAAAARNAADWLRVIRRADACWS 1ZYL Chain:A ((9-263)) ---QTLHPDTIMDALFE-HGI-RVD--SGLTPL-NSYENRVYQFQDED---R----RRFVVKFYRPERWTADQILEEHQFALQLVND--EVPVAAPVAFNGQTLLNHQGFYFAVFPSVG-GRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPT--IGL-NEYLIEPRKLFEDA--TLIPSGLKA-AFLKATDELIAAVTAH--WREDFTVLRLHGDCHAGNILWRD-----GPMFVDLDDARNGPAVQDLWMLLNGDKAEQRMQLET---IIEAYEEFSEFDTAEI----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1021 -16334 -16.00 -66.94 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.00 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: