Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSDEASRFLAE--LGIADDSVTPITGGWASWTFATGSGRILRVARNPEIDAAHRRESRLLPELAEAVSFAVPAPTHFGVH-KGMTYMVYPRLPGRGLEPG----------D-RVRAVAPMLAELHAFPVDRAAELLGCAVTAEAWRDDYLEIGKWVEGEV----LPVLDGELRDRVRREYDATLP--DLAAISPALIHRDLGCEHILTDPE--TGMPTGIIDFETATVGDPAIDYVGLLVTFGEQVTRELIAASGS----EISWRLVRFYHWLGAAHAVKYGLAERDDAIVADGIEGLRRRLPG 3TDW Chain:A ((4-298)) -NKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAISAGVP-------VTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFT-RQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRARDQDWISEGLELLEEDKA-

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1218 7956 6.53 29.57 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 6.53 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: