Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFKNNIALGKSVTEALEILERELGSQYCYCKTLEGCSNEVHIIKDVRSGLPYIM-RFTR-QSRFQNYSLEREILSHVVGRGVATSQAYLFADGIVAACIEGHCVESDKMLGDSPYYELIAKQMRRLHEISVQDDGTSV----------MYSETHYGLKSMLDISVDYIGKGRE----AELL--YKLYSEDGVLGQLVNDHP-SLLWTCISHNDLHSGNIIYSPS-AQEVRFIDWEYSTYSINAFDIACFFLEFTGIDCEISAFPCASKRQDFYLYYFGSSSP-PIDFLCLFFIPLAC---LFWAAWSSGID---G-LD--VYTKNRTRLGHATLRKLVNEIWPQCGLTLDKEDCELLELVDFTFQSLYTN 2QG7 Chain:E ((110-455)) ---------------------------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFTNGRIEEFMDGYALSREDIKN-PKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLPIFR----------------------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1219 -27775 -22.79 -99.91 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain E : 0.65 3D Compatibility (PKB) : -22.79 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: