Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRSVHDALASHPVE--TTVHRRLHDVRPHEVFEVTYD-GQRVVCKVAQHPL--GDPELEARILQFVAAETP--VPVPPIMAVGSDHFVAGWCRDLPADPT---------------VDETRSRAMGAGLARLHESASERFDAPGHLEAENGSLALDADDRWSDTLVAFLDERRRFLEPRGYGDVATEVLGLVEAFPEAFDDVGDPTLLHGNALPDHVGIDRSADPARVTRVIDFEHALVGPPAFDLLRSIGPTYGPPGATHDPDGRAAFLEGYATVRPLPA-DLEETFRPLGVVNGVAYLRALHLQRGDRDAPQSVARRARGLAEHVLEAASEVRSALRSE 5UXA Chain:A ((6-280)) --KQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNK-YAISFQAPNWIIYTEELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHT--------PEEARMSMKQRMDAVRAKFGV--GENLWNRWQAWLNDDD---MWPKKTGLIHGDVHAGHTMIDKDA---NVTGLIDWTEAKVTDVSHDFIFNYRAF-------G-EEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEF---------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1048 18300 17.46 72.62 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 17.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: