Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQSFYENTVPIAISQLRNDRQIPTLCQYFDGGQCRVFKVTFTD-GESWAVRVPLFVRHASQNTVIQLLESEARILRELEVKEFSWAARLRGCSFTFDNAIRYPFIALTWIPGSQLLWSDDFPTRPLRDKILDQVATMHISLIECSKEARGSSLKHFTRIIQNKTRRVREGLLPEITEQDCSDQMNILPNVL--LPELDEAPFAITHGDISPQNILIDAHHNVTGIIDWGFSSRVPFQQ---AACF------PRFLQLQNIDLWPNSTLQKDRETYITSVRCRAPLEVSSMMIQVLSSENADFQHCFLESIISKGMHRRLARNGWGVSS--CGQGSEGEAND--RNL------------------------ 1PHK Chain:? ((15-291)) -----FYENYEPK--------EILGR------GVSSVVRRCIHKPTCKEYAVKIID-VTGGGSFSAEEVQELREATLKEVDILRKV---------------SGHPNII-------QLKDTYETNTF------------FFL-VFDL---MKKGE--LFDYLTEK-------VTL---SEKETRKIMRALLEVICALHKLN-----IVHRDLKPENILLDDDMN-IKLTDFGFSCQLDPGEKLREVCGTPSYLAPEIIECSMND----NHPGYGKEVDM---------WSTGVIMYTLLAGSPPFWH---RK--QMLMLRMIMSGNYQFGSPEWDDYSD-TVKDLVSRFLVVQPQKRYTAEEALAHPFFQQYV

General information: TITO was launched using: RESULT: Template: 1PHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1076 -14981 -13.92 -63.21 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -13.92 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_1PHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1phk-query.scw PDB file : Tito_Scwrl_1PHK.pdb: