Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDQAFTFQTLHPDTIMDALFE-QGIR------VDSGLTPL-NSYENRVYQFQDED--------RQRFVVKFYRPQRWS-AEQIQEEHQFAHDLLDDDVPVAAPLKFNNQTLLTHEGFYYAVFPSLGGRQFEA---DNIDQMEWVARYLGRIHQTGRKEDFIARPTIG-IQEYLLEPRQVFETS----ALIPKALKDNFLNATDKLIDAVKTCWRDEISVLRLHGDCHAGNILWRD--------GPLFVDLDDARMGPAVQDLWMLLNGDKAE-----------------QRMQLETIIEAYEEFSPF-------------NSDEIVLIEPLRAMRFVYYLAWLIRRWDDPAFPRNFPWLTGEDYWRSQISTFTEQVKVLKEPPLQLTPMY 5FUT Chain:A ((7-377)) ----------RTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLG-PKLYGIF---------PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES---TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS--IEF---GYMDYAQARFDAYFHQKRKLG----------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1087 -28545 -26.26 -100.16 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -26.26 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: