Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT-G----EVEVLSGGHANVNVRLGAE---RVLRVYRRD---ASAAPKEASLLAHGWR-SFR-VPAVLSAG------------D--DFLVLEHVPHVPLQDT---AMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDA--VLDR-LAPA----LRGELSERVRAFLDANAEALRALASAPVLLHGDFKASNLHWLSS---GELLVLDWEFAYAGPGLMDVGQLLRWSPG----REFVAAFAGAYRESAPLPEEFERWAAAFDLFNLVGLLDGVEPGSRRAADVCGRISEVVER 6EF6 Chain:A ((27-309)) ---------------------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTR-PAG-FG--RFS----WDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFL-----------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1036 6669 6.44 28.26 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 6.44 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: