Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDQQQIIAVAEAVLTQRYGGQQHLTEPEEL-------SGASGTVVLRLRVANNPFLSYR-SVVVKYSPQTDDPIDDAAFLREVVAYQFTTSLSEEARPGPVMLGY-DLEQHAIILTDSGDGDTLATLLENSDEEQRIRLLRNLGTSLGKMHAGTAGAELSFNTLLARMVRARDGAREIQAMRESMLENRILDGLEIVRRAGIDVPADVADIAGTVQVHMLHGESRAFTPFDLSPDNIIFADRTQFL--DYEWAGFRDVIFDVAGVIAGFPQYISARPISEAETKVFLEAWIAEVAGVWPALQNTETLHSRI-TAALVGWAFFSVSLLYLD-GEEPRGTTRYGGGWIMADNGNYELVQDPEALEEDVERGASVLRSPAHTDFTAVEKAIRRDIGDAFSALAWYAAAGDTPRNAVIADFASHVAERLK 1LUF Chain:A ((560-860)) --------------LNPKL--LSLEYPRNNIEYVRDIGEGAFG-RVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMA-----EFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMS-----------------------PPPLSCAEQLCIARQVAAGMAYLSE----------------------------------------RK----FVHRDLATRNCLVGENMVVKIADF---GLSRNIYSADYYKDAIP------------IRWM-------------PP---ESIFYNRYTTESDVWAYGVVLWEIFSYGLQP-----YYG-------------MAHEEVIYYVRDG-NILACPENCP-LELYNLMRLC---------WSKLPADRPSFCSIHRILQRMCE---

General information: TITO was launched using: RESULT: Template: 1LUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1307 -7734 -5.92 -29.52 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -5.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_1LUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1luf-query.scw PDB file : Tito_Scwrl_1LUF.pdb: