Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTNPAAPAAPLAPPDPARIAKTVATKMPFRG-----DMTGLTPLAGDASNRRYFRIALKGT-------PSALILMQLADPEGFKKSEEAVSGASA-QIAELPFTNVLAHLQRTGVTVPQLHYYDREAGLLYLEDFGDLTLAEACRDADHARLVALYRQAIDQLVLLQVKGTKPPAPGCIAFHRRFDVPLLMWEFDHFLEYGIVARL-GQPMKPADDKAVRAEFQRIAELL--AGQSQVFVHRDYHSRNLMVDG-------SRIGIIDFQDALMGPATYDLASLLKDAYIE----------------LDDAVVDGLVDQFLDGLAAHGQ------GWADRAAFRRLFDFTSIQRNLKAAGRFVYIDRVKHNPKFLADIPRVLGYVKRNLSKYPELATLRQHLAPYVAELQ 5FUT Chain:A ((6-347)) --------------PRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNM-LFQCSLPDTTATLGDEPRKVLLRLYG-----------------GAEAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEELS-----LPDISAEIAEKMATFHGMKMPFNK--EPKW--LFG--TMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIV----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1030 -17889 -17.37 -65.29 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -17.37 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: