Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRTGDPTDAEVREIGILAGNQRATCDDRAYFQAAAERLAMLPDASEPDVPVLLLEQHYGQQG-A-LTALSSEVERTFDARLADGRAFILKTSSRPQ-ALESFQFQSAALAGLRG-ADGVMAPKVIDTLSGGLMFE-----HEGGCGYLQTRMDGIPLHMVPRTPEILREVGAALARLNLAMKDTDPPAARRPVLWNIACWP----WLVELERYL-SKGRTAKLVRSAMAEYIRDISPH-IADLDWQVTHNDPSPFNMIDT-G---KGIGFIDFGDGGWNPRVQDLAIAAGHFVIDPRTPLGGAEHVIAGYASLAPLSELEASLLLGLMRARQSALVLINNWRSHLFPAAAPYIMKNVKRAEQGLAVLALLDVSSGEAAIRAAAGLDPP 6EF6 Chain:A ((27-320)) ------------------------------------------------DVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDS-DVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD-PRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGS-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 -620 -0.50 -2.28 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -0.50 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: