Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MSINFSNLSKNI-----------NFINFSKISRGNNKELKYIVKDSE-MNRYLFKINS------IIHLDNKVNEFRFAKRIEHLPFEKSEIVDLCVFNQNRYICSIYKWIDGKILEDQIK---NYPSNKQYSFGFKAGRSLKLIHDLPIRDEELKQVEELPVRKIIEYYHQNKRFENDNIIIDFILKNNSKIKDEKICFLHGHYSIKNFIIKNNDEISLIDYNQCYFGDYVQDFSKNEIYNSYYSEQ-FAQGIIDGYFYNRKIPDTFWLKYAYYAAYNCITLFLWASKQEVISTKIKIKEMIQNVLNQFNNFESIIPNWYKKNVLSKERQ--------KFINSPYTLEKILGTP-------------------KKENKNDSKIKYFDEMYDDNDDDLLDEKMMDSFKEFLKNVAKK 1APM Chain:E ((10-350)) SEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTG-SFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFL----VKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFAEPHARFYAAQIVLTFEYLHSLDL--------------------------------------------------IYRDLKPENLLIDQQGYIQVTDFGFAKRVKGR-------TWTLCGTPEYLAPEIILS--KGYNKAVDWWALGVLIYEMAA---------GYPPFFADQPIQIYEKIVSG----KVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF-------

General information: TITO was launched using: RESULT: Template: 1APM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1376 44130 32.07 155.93 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain E : 0.64 3D Compatibility (PKB) : 32.07 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_1APM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1apm-query.scw PDB file : Tito_Scwrl_1APM.pdb: