Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTVAAPAPALAEVACFSRDLCKELVK-EWSSLDSSCFSISTVSGGITNMLLKVSVKEG----SDSESSVTVRLYGPNTDLV--IDRERELQGSGLGYLEYLRMEWFRHSSMLGHYHLQVYTQRLLFCTHRCMETGEPAINASANNMKEPRIAAEIAKQLQKFHQVDIPGSKEP-QLWNDIFKFLKKASVLKFEDNEKQKRYETISFREIQDEVKELKDLSDLLHAPVVFAHNDLLSGNLMLNDL-----EEKLYFIDFEYGSYSYRGFDIANHFNEYAGFEC---------DYNLYPDKDVQYHFFRNYLSDRPSELKNRLFASICEKRNVGYLAFVQYKVQEQDLDDLYTETNTFRLASHLYWALWALIQARVSPIDFDYLGYFFLRRHVVPNRKLYNL 5FUT Chain:A ((5-376)) ----------EPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFA-----ILAERSLG-PKLYGIFPQG------------RLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFEN-----------L------STEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKL-

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1372 17622 12.84 53.89 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 12.84 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: