Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCYGSMSCNPVEQRGEELKYMEKLIELYAQWAGEKPSTIEKLEGAGSNRVYYRFGN----KSGQSVIAVIGTSRDENHAFIYLTEHFTDKQLPVPQILAVSDDELRYLQTDLGHTSLFDMLKSARDAGGRYTQKEKQLLTKTLQALSEMQVRGAVGLDFQQCYPQPEFNVESVLFDLNYFKYCFLKATGIDFHELMLEANFRMFAKDLTAEPAECFLYRDFQARNVMLDNDGNPYF-----IDYQGGRKGPYYYDLASFLWQASAK-----YPFKLRRDLVSAYYESLKQYTEVPSSRHF---VERLNL-----FVLFRTLQVLGAYGFRGYFERKKHFIQSIPAAMQNLRELIKLGEHVFPYPYMMSVLKEMTELPQFANKEERKVKRTDGFKITDNNIFKANPVDGPATFSKYDDKGELIVRVNSFSYHKGIPEDPTGNGGGYVFDCRSTHNPGRYEPYKNLTGLDEPVIRFLEDDGEILVFLESVYQLADRHV---ARYIERGFTSLIFSFGCTGGQHRSVYCAQHLAEHIHDKFGIEVRINHREQKIEQTLEAK----------- 1TF7 Chain:A ((14-497)) ------EHQAIAKMRTMIEGFDDISH----------------GGLPIGRSTLVSGTSGTGKTLFSIQFLYNGIIEFDEPGVFVT--FEETPQDIIKNARSFGWDLAKLVDE-GKLFILDASPDPEGQEVVGGFDLSALIERINYAIQKY-------------------RARRVSIDS------VTSVFQQYDASSVVRRELFRLVARLKQIGATTVMTTERIEEYGPIARYGVEEFVSDNVVILRNVLEGERRRRTLEILKLRGTSHMKGEYPFTITDHGINIFPLGAMRLTQRSSNVRVSSGVVRLDEMCGGGFFKDSIILATGATG-----------TGKTLLVSRFVENACANKERAILFA--------------YEESRAQLLRNAYSWGMDFEEMERQNLLKIVCAYPESAGLEDHLQIIKSEINDFKPA--------RIAID--SLSALARGVSNNAFRQFVIGVTGYAKQEEITGLFTNTSDQFMGAHSITDSHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKAIREFMISDKGPDIKDSFRNFERIISGSPTRI

General information: TITO was launched using: RESULT: Template: 1TF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2494 57217 22.94 127.72 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 22.94 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_1TF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1tf7-query.scw PDB file : Tito_Scwrl_1TF7.pdb: