Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MSVYTPLSLDEVRTFAAPYG------LEVLELNPIQGGIQNTN--YFLVDVNRKQYVLTVFEELDAQGAGELIPVLEQLGTHDVP-------VAVPLKHSGQAVHFIAGKPAQIAPRLMGHHPMQTTVAQVAAIADAQAKLHVALQDFPLEREYRRDHQY--WTGVAEQLKPNMTQDDQTLLEQVYQAFNAKTAQYSNRPTGFIHSDLFRDNTLFEGE--QLQGILDFYELNQDELLFDIAITINDFCT-------EYPAAHLNSDKVGAYLEAYQKVR--ALTSDELECLD----VFLAMAACRFWSMRLQ----VAQKNKEEGRTGDDILQKDPQEMRAMMQDRLSHVKA----- 4OCV Chain:A ((24-378)) TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQ------YAVVMDGLRDGSI-PLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1479 30572 20.67 98.94 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 20.67 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: