Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MNSYAHLNHYISP------SAEAGLSDAWRRSLGRLGDYSAILIRSLNLRPLEVSLHDQTEEHILVRIRLPGSYLILRVAPEDDLAAYVY-----QIRTLNGHNIPAPQIIQRDLS-RSLVPFAFTLESYVPGVTAIELQSAPLLRGAGRQAGRALRRMHRIPVTSAGRPTAS--GRWTQQNWRTVLRQIGRRLAAPPADALIFGERE-----QRVVADLLDHPVL--------------ECQQPSLIHGRFSPRAVRCTSSGN-----VHVEALIDPGHY--VG----GDGLYDLAC-----GLCAAYPEPW---------REGL-------LDGYQSAGPLNEAERRRLPLLQLLTNYWEACRLYMHAEPHE------AARSEAIRLMEEQVGLTRLVSV--------------------------------- 4C2E Chain:A ((44-478)) ADSERDKAMDKIEKAYELISNEYVEKVDREKLLEGAIQGMLSTLNDPYSVYMDKQTAKQFSDSLD-SSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQRPGTKK---QLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTTEDFAKALRELEKK----EIEGLVIDVRGNPGGYIQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSAAASEILAGALKEAGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGIIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLF

General information: TITO was launched using: RESULT: Template: 4C2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1577 70822 44.91 219.26 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 44.91 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.187

(partial model without unconserved sides chains): PDB file : Tito_4C2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4c2e-query.scw PDB file : Tito_Scwrl_4C2E.pdb: