Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLSVLFEQISPYLPPQEALKVENIEDGYSNHVMKLHWY--NAPRAVLRVPMLDTEAFLIDRHAELNALKAAQNAGISPTVIWSNDQGAILSEYIHAPALSWDVKHNSRDIKRIGALFTQVHALSVNAPSR---------------NIYQVIEHYVGGIEHHD--TN---G--------AHKHEVAYLQHCFDQMNKVDSRGRPDVLCHNDLNPKNVLLNN--EEIWFIDWEYTALGDPLFDLAAVSRSH---------NLTLDQQKILIEAYDDTLNV---QDTLKTIHDYGFAYALREMAWLLLKHVISVDDHQSLDYYREFKDTKKLNPFI 2QG7 Chain:E ((111-449)) --------------------LEFQIING-ITNILIKVKDMSKQ-AKYLIRLYGPKTD-EIINREREKKISCILYNKNIAKKIYVFFT-NGRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCK---RENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHS-----S-DFDFINYGMTRLTAS-

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1007 -5 -0.00 -0.02 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.64 3D Compatibility (PKB) : -0.00 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: