Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHEKLKQLSPLLKQAISFQPMTSGFSGDRTFLVT--TPSEKLVLKLSDIQK-YASLKKKTSILQNLKKRGVLCSEVMEVGVSAEQNCTFRIFPFIEGENARASIRL---LTEEEQYKIGRRAGRDLRIMHTCDAPTYVAPWDDRVLAKHERYVYAYQSSGVAFSHDQFVLDFIQSHADVVKERPNQFQHDDFHVGNIIIQRHQYAGVIDFDQSDWGDPIHDFYKLSLFSRESSIPFSAGQLDAYLSTQTTHDFWLLFSIYTAMSCFSSIVWTLSYDPGHVKDMVNRVERMLTDHKRFTQLTPVWYTDWLDSCV--------------------------------- 1IA8 Chain:A ((2-276)) --------AVPFVEDWDLVQTLGEG-AYGEVQLAVNRVTEEAVAVKIVDMKRCPENIKKEICINKMLNHENV----VKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIG-----------------------------------------------------ITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLN--KMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDS-------APLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRP

General information: TITO was launched using: RESULT: Template: 1IA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1077 -1706 -1.58 -7.32 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -1.58 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_1IA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ia8-query.scw PDB file : Tito_Scwrl_1IA8.pdb: