Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTKPLSILQPGTGLFNTHVTL-----EDVNKAIKEQMSTESELTAESKMEVIGEGNGFSSCVILITCHWTI----------PSSHLPKKLILKIVSFV------HVQGLLNKSNEEGNSVMSPEEEAHVHAHFEKSCQKGHNLEVEFCEAFGHLEGLLLPKVFFSQKFEEDNPNKGFV-GMEFVEGSVVRHCYENVTVDELQPILKALARLQALSLSTESCRNLDNGEAFEESLMDMLSEDG-LKGIFDQSRNIDQKLSE---KVERIEQNHKEILNLETVLNL----NKVVGIDQKVICHGDLWAANILWTQTDGGFIADKVLDYQESHMGNPAEDLVRLLVSTISGADRQSHWEHILEQFYTYFTDEIGSNNAPYTL--EQLKTSFKLYFPV----------GALTLISLFGPAVDMKLQGMESGKAENYRRIVIEKVDCLLDDVL--NFHDFNKKFTGKN---- 5L3T Chain:B ((1-455)) MDMANQLLDELAHGNFS-HLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSF-HLQL-----HTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEY--QLDQQIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTT----EWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

General information: TITO was launched using: RESULT: Template: 5L3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1707 58048 34.01 142.62 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 34.01 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_5L3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5l3t-query.scw PDB file : Tito_Scwrl_5L3T.pdb: