Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLDSPGTLKQCLISQRPLQGGLTHDNTLLICAD--------GSQWVCKRSLGRHQ--SGLATEANVYSLLVNTRFALPIKLVHLDIASQMLLRPYIEGTTTTSWA---QLSIEQAILLLKAIHQITATHHGKVSSPTSLTLNPANQLRVIWRYFRSRT-SQ---RAKMM-DIYGKLSTILQNRQSLFN-TLSTFTLNHGDFHPGNLIATPA------QQLVPIDWERAHFGDPAFDLALINWHGQDP---------------IVNSALQARAIALYTQCPAE-------------QVALQKRVVCWSLVRLFNDYLYLTSNGLKVDKLAHFEETTAMLLNAAS 5FUT Chain:A ((30-351)) -----------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----GAEAMVLESVMFAILAERSLG-PKLYGIFPQ---GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKW-------LFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKI-------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 843 -22249 -26.39 -90.44 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -26.39 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: