Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAFEKIPAFQQWQKVEAIHRGWSTDLKFKVTKNQETFLLRIFQQEE-LLAKQQEYQFIKKVAALG--FPSSKPFLCAPIPESEQGYMLLTYLEGEDLSDV-LPALSPKRQLNLGVEAGRYLNKIHKLLLPERISQ------REIAQNLYEKKQSQLNKYKES-QFCMPY---QQPIISYLEKQLPLLQQRPVVYQHGDFHVGNFIYLPTRQVGVIDFNRWDFGDPYEEFYKLQFFSR-NVSPLFAYGQLQGYFAGKVPTLFWQFQKLYTFHAGLYSLVWALSFGEKEIRTMEQQYQQLLEDYNCGELLVPKWFSTIQRKGLRF 3HAM Chain:A ((14-279)) --------KQIKINELRYLSSGDDSD-TFLC---NEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQ------SDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDK-FLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAV-LFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHK-APEVAERKAELNDVYWSIDQIIYGYERKDR-------------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1053 19138 18.17 76.25 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 18.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: