Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGHGMAGDDWQYRKTMPQNILRPTVEAALPHVAGAEWEPLAGGRTNPVFKIN-A--DAPLVCKLSCLSGETPLFGNRALDEAAILKHLEGSGLAPSLTDVLAVPEGEILIYSFVEGDILTQ-------TIPAAVRILRQVHQAEIPDNLPR--------LDGSSAAIIQKTKAILETLPVQ------------K----A-----M-AISFAEPKVELPDTLVPRLIHGDPVPANFIQSK-GKLTLIDWQCPAIGDPCEDLAIALSPAMNAVYG-DGPLTPQQMKECLNAYGD-RT------VSDRYRKLAPLFHWRMAAYCAWKVEQGD-KDYAAGVKLELAALK 2QG7 Chain:D ((110-448)) ----------------------------------SLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGP--KT-D---NREREKKISCILYNKNIAKKIYVFFT---N-GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVES-LCK-RENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTA--

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 961 -9776 -10.17 -39.58 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : -10.17 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.360

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: