Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGAAEIIEAILGESGPDYESYSFEETPGDTRSDSHFVTVEYQGESYEVVVKLAPEVDSTFAVEPFLHEYVAERTDVPLPGILVFKEEPEQDVPPYFITERIHGDNLDEHFESFSMDERGAIMFEIGEILGDMHSTIAFEGYGRLDLDDGRLIVRDLSWDWRAYFEELTQGHIDQLAETTFEDLQPTARERLADRLSVVPKQGTPRLVHDDFRPGNLLIEPDGPEISAVLDWEKTLAGDPLYNLAQIELLFIDSVFRDPDEREQLRERLYEGYGTETDFDADESYQACKPLYQFSTLVWRMAGFDSIYGDASPLARTRAEAYYREQFTNLARTLEPDR 1J7L Chain:A ((2-264)) --AKMRISPELKKLIEK----YRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHD---GWSNLLMSEADGVLCSEEYED--EQSPEKIIELYAECIRLFHSID----------ISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFV--KDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEE-----QYVELFFDLLGIKPDWEKIKYYILLDELF----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1J7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1274 -75188 -59.02 -285.88 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -59.02 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_1J7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7l-query.scw PDB file : Tito_Scwrl_1J7L.pdb: