Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MLYTLGQKIGEGGCSEVFEVGDNRIIKL-AKENTSFEALRREYLNNCIAWECGLPIPQPFELTKMSGRPGIVFEHIAGKTMMERFFNQVIVSHNNEVKQ-ADVQLTAQLLFRVHQALAEGKELPCQKSIIK-SNILSVNYLSASE-----------KESAISLLESLETKQ-CLCHGDPNPRNVIVKDD----GEAILLDWMNATIGNPEADIAEYI------VMIRYAVL-PSSFPEETR----------DMFDSIRE-DLIDIFIDEYYRLSGISYDDVVPWIIPILARKLSADAISDVEKKKLVAEIRRDLGKQKNRV-- 3CSV Chain:A ((4-333)) SREDEIRDFLATHGYADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAP---EIYAEEHARGLLLIEDLGDALFTEVINN------DPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASA-AEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1243 2102 1.69 7.67 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 1.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: